Product Uses Include
Immobilizing tubulin onto a solid surface via streptavidin.
Creating tubulin or microtubule affinity matrices with streptavidin coated sepharose beads
High through-put screening using a proximity assay.
Microinjection into cells followed by electron microscopy of streptavidin conjugated gold particles to determine the cellular localization of the tubulin.
Porcine brain tubulin (>99% pure, see Cat. # T240) has been modified so that random surface lysines contain a covalently linked, long-chain biotin derivative. A long-chain biotin derivative was selected for this procedure because it allows the biotin molecules to be spaced far enough away from the tubulin protein so as not to interfere with its activity, e.g., ligand binding to SPA beads or other streptavidin based reagents. Biotin labeled tubulin is supplied as a lyophilized powder.
Cytoskeleton, Inc. also offers biotinylated cancer cell tubulin (Cat. # H003 )