Identification and characterization of FtsZ binding proteins
Characterization of FtsZ dynamics
Developing FtsZ ligands that may be used as anti-bacterial agents.
FtsZ is a bacterial cytoskeletal protein that is essential for cell division many prokaryotes (1). It has been shown to be a bacterial homolog of eukaryotic tubulin, based both on a low sequence identity and a striking structural similarity (2). Just like eukaryotic tubulin, FtsZ polymerizes as well as binds and hydrolyzes GTP in a polymerization dependent manner.
Staphyloccus aureus FtsZ protein has been purified after over-expression in E.coli. S.a. FtsZ has a 6xHis tag and an approximate molecular weight of 55 kDa (Fig. 1). FTZ02 is provided as a lyophilized protein with buffer and stabilizers.
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 10% polyacrylamide gel. ?FtsZ protein was calculated to be >90% pure (see Figure 1, Lane 2).
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >90% pure. No free dye is apparent in the final product.
Figure 1: A 100 μg sample of S.a. FtsZ protein was separated on a 4-20% gradient SDS-PAGE gel and stained with Coomassie blue. Lane 1, 100μg FTZ02. SeeBlue molecular weight markers are from Invitrogen.